Re: dessicant capsules
From: | Donna Simmons <dsimmons@usc.edu> |
I use them to store dipped autoradiographs (emulsion must stay dry
during exposure), and frozen sections [fixed or unfixed] on slides
saved in the freezer or refrigerator for immuno or in situ
hybridization. The plastic slide boxes are sealed for air-tightness
with 'stretchy' black electrician's tape, and for light-proofing with
aluminum foil if needed. In addition to keeping tissue very dry and
thus preventing any unwanted chemical reactivity, the dessicant
prevents condensation of moisture from the atmosphere on slides as
they warm to room temperature in preparation for further processing.
Note: when storing frozen tissue blocks for future sectioning, the
opposite problem is encountered -- the goal is to prevent dessication
of the block and resultant gummy or rubbery tissue. In that case I
seal the block airtight with some water ice included, so that
sublimating water vapor is in balance with the air surrounding the
tissue.
-D
>I have never heard of anyone using these things to store slides. What is
>the purpose? Is this something recommended for long-term storage? I
>would be interested in a vendor if you(s) think this is a good thing to
>use.
>
>Thanks,
>
>Trisha
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