Kenneth

What are you fixing and how big is it?

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Wed, 28 Feb 2001, Kenneth Wester wrote:

> Hi All.
> 
> Is there anyone who can give me informatiom on how to properly use the
> Zamboni-fixative on cryo-sections? In my hands, the immunoreactivity is
> preserved excellently, e.g., for EGF-receptor staining, after Zamboni
> fiaxation. However, the overall morphology is poor, even when compared to
> fixation in aceton and/or methanol.
> 
> I have tested air-drying the sections for between 30 seconds up to 1 hour,
> without any major improvement. Usually I fix for 2-3 minutes followed by
> rinsing in distilled water.
> 
> Greatful for any tips regarding the morphology problem,
> 
> Kenneth Wester
> 
> Kenneth Wester, Ph.D.
> Depts. of Surgical Sciences and Genetics & Pathology
> Experimental urology, The Rudbeck laboratory
> Uppsala University
> Dag Hammarskjöldsväg 20
> S-752 37 Uppsala, Sweden
> phone:  +46 (0)18 611 02 06
> fax:    +46 (0)18 471 3432
> e-mail: kenneth.wester@genpat.uu.se
> 
> 
>