Re: IgG, IgM on FFPE tissue
|From:||Katri Tuomala <email@example.com>|
What are you looking for in these monkey tissues? Plasma cells, lymphocyte
surface antigens or immunoglobulin deposits in vessel walls or basement
membranes? Or something else? Katri.
Hamilton, Ontario, Canada
> Hello Histonetters,
> we try to do direct immunofluorescence on paraffin sections of monkeys with
> Dako antibodies for IgG and IgM. Until now we had no success with HIER or
> enzyme digestion with trypsin. We'll go for their recommended pronase
> treatment before the staining and we are hoping ....
> But now I would like to know from you experts out there: any
> recommendations? How about procedures for human FFPE tissue ??
> And again thanks a lot in advance for all your comments and ideas.
> Have a great time,
> Antje Marcantonio
> BU Transplantation
> Novartis Pharma AG, Basel
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