Re: IgG, IgM on FFPE tissue

From:Katri Tuomala <>

Hi Antje,
What are you looking for in these monkey tissues? Plasma cells, lymphocyte
surface antigens or immunoglobulin deposits in vessel walls or basement
membranes? Or something else? Katri.

Katri Tuomala
Hamilton, Ontario, Canada wrote:

> Hello Histonetters,
> we try to do direct immunofluorescence on paraffin sections of monkeys with
> Dako antibodies for IgG and IgM. Until now we had no success with HIER or
> enzyme digestion with trypsin. We'll go for their recommended pronase
> treatment before the staining and we are hoping ....
> But now I would like to know from you experts out there: any
> recommendations? How about procedures for human FFPE tissue ??
> And again thanks a lot in advance for all your comments and ideas.
> Have a great time,
> Antje
> Antje Marcantonio
> BU Transplantation
> Novartis Pharma AG, Basel

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