Re: IgG, IgM on FFPE tissue

From:DDittus787@aol.com


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Direct Immunoflourescence works best on fresh tissue, not fixed, however if 
you want to do those antibodies on paraffin embedded tissue you must use 
either a dab or aec detction kit to see the markings and I warn you (from 
previous experience with both ) look out for high background , the dilution 
factors for both methods will be greatly different. Good Luck.
                                                   Dana

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>Direct Immunoflourescence works best on fresh tissue, not fixed, however if 
<BR>you want to do those antibodies on paraffin embedded tissue you must use 
<BR>either a dab or aec detction kit to see the markings and I warn you (from 
<BR>previous experience with both ) look out for high background , the dilution 
<BR>factors for both methods will be greatly different. Good Luck.
<BR>                                                   Dana</FONT></HTML>

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