Re: Composite blocks.

From:Amos & Theresa <atbrooks@snet.net>

Hi
    We have done this in the past, and with the sudden interest in
micro-arrays, I am seeing A LOT of this lately. The tissues should stain
fine just be aware that some tissues are prone to different problems,
similar to the differentiating of the trichromes you are used to. Keep
in mind that some tissues (liver and kidney) have lots of endogenous
biotin so expect background staining on them when the rest look great.
Hematopoitic tissues (bone marrow and spleen) have lots of endogenous
peroxidase, so expect similar problems there. Also there is the obvious
fact that not all tissues will react with the same antibodies. Of course
you will not see gastrin in bone marrow (hopefully). That having been
said These multi tissue blocks usually work well, and are fantastic for
validation of new antibodies.
Amos Brooks

"Dr. Ian Montgomery." wrote:

> In the past and mostly for undergraduate teaching, I've prepared
> composite blocks of various tissues. You know the sort of thing,
> pieces of kidney, liver, muscle, gut, lung and brain sandwiched
> together and stained using a variety of techniques. Works well,
> although trichromes can be a bit of a bugger when differentiating. For
> teaching very handy as the students can move from tissue to tissue
> without changing slide when comparing the structures.
> Now, my latest problem and hopefully easily solved. Has anyone tried
> this sort of thing for IHC. I have 9 pieces of tissue from a very
> large number of animals which if I can prepare composites blocks off
> would make my life a lot easier. Does IHC throw up any cheeky wee
> problems which I've not thought about, eg. tissue dependent retrieval,
> or is it pretty straight forward.
> Comments very welcome, sorry I can't be cloned, grow extra hands or
> work double, triple shifts.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
> Tel: 0141 339 8855.  Extn:6602.
> Fax: 0141 330 2923
> e-mail: ian.montgomery@bio.gla.ac.uk




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