Re: A.Rat TGF-beta

From:Greg Dobbin <dobbin@Upei.CA>

Hi Mermina,
Do you *know* for a fact it will only react with human TGF? Or, has it 
just not been tested with rat? Sometimes if a company does not 
know if their Ab will cross-react (ie has not been tested) to other 
species they will cut a deal of some sort with you, if you are willing 
to share information about what you find regarding cross-reactivity 
to other species.

You didn't mention which companies you tried. Did they include 
Cedarlane, Serotec, Bethyl, BD Pharmingan, Novocastra, (to name 
the ones I frequently try for animal/veterinary specific Ab's). Maybe 
someone else will have more specific info for you.
Good luck.

Date sent:      	Mon, 26 Feb 2001 10:46:15 -0500
From:   (Hermina Borgerink)
Subject:        	A.Rat TGF-beta
Forwarded to:
To:             	Histology listserver <>

> Good morning Histonetters,
> I am trying to find an antibody for TGF-beta that can be used on
> demineralized and paraffin embedded rat tibias.  I have called several
> companies that have TGF-beta antibodies available, but none of them can
> be used on paraffin embedded tissue, with the exception of one, but it
> cross-reacts with human only.  Any help would be greatly appreciated.
> Hermina
> --
> Hermina Borgerink, BA, HT(ASCP)IHQ
> Department of Pathology
> Wake Forest University School of Medicine
> Winston-Salem, N.C. 27157
> PH (336) 716-1538
> Fax (336) 716-1515

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