Re:

From:Roger Moretz <stamptrain@yahoo.com>

John:
It is difficult to comment, since I don't have any
idea what your current issues are, or what it is you
need to improve.  I am surprised to hear that you
expect "better infiltration", since Spurr's is a very
low viscosity resin.  Also, do you remove the resin
prior to staining?  There was a thread on histonet not
that long ago about the use of MMA that you should
read.  I know that many of the "bone" labs use MMA, so
any one of them can probably provide a more on-point
response than this.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
--- "Bobst, John" <BobstJ@mail.medicine.uiowa.edu>
wrote:
> 
> I am currently switching my embedding medium from
> Spurr's plastic to
> methylmethacrylate.  I have been told the
> infiltration is much better and I
> will receive much better results.  I would like any
> comments or suggestions
> about the problems I may encounter or the
> assumptions I should not make.
> Also, if anyone has a MMA protocol for embedding
> times, I would be very
> appreciative to borrow it from them.  Any additional
> information is
> definitely appreciated.
> 
> Sincerely,
> John A. Bobst
> 
> University of Iowa
> Bone Healing Research Laboratory
> Iowa City, Iowa
> 
> (319) 335-4377
> 


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