|From:||Roger Moretz <email@example.com>|
It is difficult to comment, since I don't have any
idea what your current issues are, or what it is you
need to improve. I am surprised to hear that you
expect "better infiltration", since Spurr's is a very
low viscosity resin. Also, do you remove the resin
prior to staining? There was a thread on histonet not
that long ago about the use of MMA that you should
read. I know that many of the "bone" labs use MMA, so
any one of them can probably provide a more on-point
response than this.
Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
--- "Bobst, John" <BobstJ@mail.medicine.uiowa.edu>
> I am currently switching my embedding medium from
> Spurr's plastic to
> methylmethacrylate. I have been told the
> infiltration is much better and I
> will receive much better results. I would like any
> comments or suggestions
> about the problems I may encounter or the
> assumptions I should not make.
> Also, if anyone has a MMA protocol for embedding
> times, I would be very
> appreciative to borrow it from them. Any additional
> information is
> definitely appreciated.
> John A. Bobst
> University of Iowa
> Bone Healing Research Laboratory
> Iowa City, Iowa
> (319) 335-4377
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