RE: neonate mouse bones
From: | Patsy.Ruegg@UCHSC.edu |
If you can cut your samples without decal that is what you should do, you
can always put the cut sections in EDTA just before IHC staining to ensure
all the calcium has been removed which will cause problems with IHC
staining. I do chondrocyte markers all the time on rat tibia and embyro rat
tissue, if I decal I use formic acid, it is good for IHC (Immunocal from
Decal Chemical).
Patsy Ruegg
-----Original Message-----
From: Karen Glyde [mailto:klg2@psu.edu]
Sent: Tuesday, February 20, 2001 1:04 PM
To: histonet@pathology.swmed.edu
Subject: neonate mouse bones
Hello all, I need to do IHC on neonatal mouse bone days
4,13 and 18. The
particular interest is in the epiphyseal plate of the tibia
(chondrocytes) The info I have rouned up suggests acid
decalcification is
not appropriate when followed by histochemical techniques
and
investigations but rather the use of citrate buffer (pH4.5)
after a 24 to
48 hour fix in 80% cold alcohol. OR is bone this young
still cartilaginous
enough not to need decalcification? Reactions?
Suggestions? Has someone
done this?
Thanks,
Karen L.Glyde
Electron Microscope Facility
Life Sciences Consortium
Pennsylvania State University
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