RE: Vacuum sealing for tissue storage

From:Roberta Horner <>

We seal all of our tissues after they have been cut in.  I put the tissues
in the bag (we use the heavy duty) and just enough formalin to cover them
then I work all the air bubbles out of the bag and seal.  Most of the
samples are fine months later but I have found some have dried.  We keep the
tissues for 6 months then discard.  I have had a few leak but you just have
to make sure that you seal the top well.
Roberta Horner HTL
Animal Diagnostic Lab
Penn State

Hi to all,

First, I want to thank those who replied to my lipid staining question.  I
still haven't found the answer I was searching for but I now have a few new
resources to examine.  Thank-you....

I have a new question.  Does anyone use vacuum to seal residual tissues in
plastic/mylar bags?  If so, have you ever had any trouble with tissues later
on. Have you ever had leakage problems?  How do you prepare the fixed tissue
for vacuum sealing (tissue in formalin, formalin soaked gauze around the
tissue, etc.)?  Have you ever gone back years later to work on these

Thanks in advance for any info sent my way.

Judi Ford
Palo Alto, CA

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