IgG, IgM on FFPE tissue


Hello Histonetters,

we try to do direct immunofluorescence on paraffin sections of monkeys with
Dako antibodies for IgG and IgM. Until now we had no success with HIER or
enzyme digestion with trypsin. We'll go for their recommended pronase
treatment before the staining and we are hoping ....
But now I would like to know from you experts out there: any
recommendations? How about procedures for human FFPE tissue ??
And again thanks a lot in advance for all your comments and ideas.
Have a great time,


Antje Marcantonio
BU Transplantation
Novartis Pharma AG, Basel

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