EDTA pretreatment - sections falling of

From:Tibor Ric <tibor@home.se>

Hi again!
In my previous letter I've forgotten to tell You that we cook in the
microvawe but not under pressure...

Hello Everybody!

We at our immunohistolab have started to use EDTA pretreatment (1mM; ph8,0;
4x5 min) for some antibodies. Our big problem is that  some sections
(expecially breast sections, cytospin materials and smears) all falling of
the glas...

Any suggestions to avoid the problem without chaning the buffert?

Thanks in advance!
Tibor Ric, Laboratory Scientist
Immunohisto lab at Karlstads hospital in Sweden.

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