EDTA pretreatment - sections falling of
|From:||Tibor Ric <email@example.com>|
In my previous letter I've forgotten to tell You that we cook in the
microvawe but not under pressure...
We at our immunohistolab have started to use EDTA pretreatment (1mM; ph8,0;
4x5 min) for some antibodies. Our big problem is that some sections
(expecially breast sections, cytospin materials and smears) all falling of
Any suggestions to avoid the problem without chaning the buffert?
Thanks in advance!
Tibor Ric, Laboratory Scientist
Immunohisto lab at Karlstads hospital in Sweden.
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