Composite blocks. -Reply
|From:||Tony Henwood <AnthonyH@chw.edu.au>|
As long as the tissues were fixed for equivalent times and be equal in
thickness I can't see a problem with the IPXs. I use composite blocks of
liver, skin and gut for my keratin controls.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
>>> "Dr. Ian Montgomery." <email@example.com>
1/March/2001 01:08am >>>
In the past and mostly for undergraduate teaching, I've prepared
composite blocks of various tissues. You know the sort of thing, pieces
kidney, liver, muscle, gut, lung and brain sandwiched together and
using a variety of techniques. Works well, although trichromes can be a
of a bugger when differentiating. For teaching very handy as the
can move from tissue to tissue without changing slide when comparing
Now, my latest problem and hopefully easily solved. Has anyone
tried this sort of thing for IHC. I have 9 pieces of tissue from a very
large number of animals which if I can prepare composites blocks off
make my life a lot easier. Does IHC throw up any cheeky wee problems
I've not thought about, eg. tissue dependent retrieval, or is it pretty
Comments very welcome, sorry I can't be cloned, grow extra
or work double, triple shifts.
Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Tel: 0141 339 8855. Extn:6602.
Fax: 0141 330 2923
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