ACHE staining for Hirschsprung's disease ?IPX instead

From:Tony Henwood <>

Dear fellow histonetters,

Does anyone use the monoclonal antibody Clone HR2 to demonstrate
ganglion cells in formalin fixed paraffin sections?
I would be interested to know whether any labs prefer to demonstrate
these cells using IPX on formalin fixed paraffin sections rather than
ACHE staining on frozen sections and what antibodies they use
(?synaptophysin, ?Cathepsin D, ?others).


Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

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