Sabrina Dize here, for those of you who remember I'm Morgan's mom. She is
improving although it is thought that she will always be a very young child
mentally. She is quite happy and enjoys people and school. I would like to
thank you again for the prayers and gifts that where sent. If anyone wishes
I will send them a picture via e-mail.
On to my question. Has any one every experienced "smudged or watery"
looking nuclei? They almost look as though someone randomly erased them. It
occurs most often in our biopsies but has occurred in larger tissue as well.
slides are cut at 4-5 microns
dried for 15-20 minutes at 67 degrees
allowed to cool 5 min
placed in xylene for 5 min
placed on hacker linear stainer
set up follows:
each bucket has same time of 1 min 20 sec with a drain time of 5 sec
Richard Allen 7211 hematoxylin
" " "
" " "
Richard Allen clarifier II
Richard Allen bluing
Richard Allen eosin/phloxine
when they come off the stainer they are either coverslipped immediately or
placed in xylene.
Sakura coverslipper (tape)
all alcohols are changed daily, one hematoxylin is rotated daily, xylenes
are rotated, clarifier and blue are changed daily, eosin is changed daily
We don't think that it is occurring during processing because some original
cuts may not have it but it shows up in recuts or visa versa. this has
really got me puzzled.
it's not limited to one particular microtomist or microtome
not any one surgeon
not a particular day of the week
not a particular time of day
oven temp is stable at 67 (for 30 years this oven was set at 74C without a
today I tried a different blade- still there
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