More information on our Gram Stain problem:

1.  I have tried the "standard" gram method (Gram, 1884), the Gram-Twort method (Twort, 1924) and Lillies modification of Gram's Stain (1965).

2.  I use Twort counterstain for all of these, as this is the Pathologists' preference.

3.  Gram -ve's stain fine, however gram +ve's are virtually indistinguisable from the -ve's - ie all the bugs seem to stain pinkish.

Any ideas as to the source of my problems?

Thanks again in advance,
Aidan


_____________________________________
a i d a n   c   s c h u r r
 section head, histology
  department of pathology
hutt valley health
high street, lower hutt
wellington
new zealand
telephone ++64 4 5709173
facsimile ++64 4 5709214