Dina,
Here is the method we use. Doing a Hx and a Nuclear fast red stain
seems odd, since they are both nuclear stains???



KERNECHTROTT (NUCLEAR FAST RED) COUNTERSTAIN
NOTES:
Kernechtrott is an anthraquinone dye.  When complexed with aluminium,
it has excellent light fastness and does not require differentiation.
SOLUTION:
Bring 100ml of 5% aqueous aluminium sulphate to the boil. Remove from
heat and add 0.05g Kernechtrott (calcium red, nuclear fast red CI60760). 
Boil until no more colour change is noted (approximately 5 to 15 minutes).

STAINING:

1.	Deparaffinise and hydrate slides
2.	Stain in Kernechtrott 2min.
3.	wash in water.
4.	dehydrate, clear and mount.
REFERENCE:
Waldrop, F.S., Puchtler, H., (1977).  Stain Techn., 52(4);237.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital Westmead,
Locked Bag 4001, Westmead, 2145
Tel: (02) 9845 3306
Fax: (02) 9845 3318


>>> <dgaupp@tulane.edu> 9/February/2001 05:01am >>>
Hi Histonetters,

Does anyone out there is histoland have a protocol for nuclear fast red
for 
paraffin?  Also is there a protocol for hematoxylin and nuclear fast red
stain 
together(instead of H & E)?

Thanks,

Dina