nuclear fast red stain -Reply
|From:||Tony Henwood <AnthonyH@chw.edu.au>|
Here is the method we use. Doing a Hx and a Nuclear fast red stain
seems odd, since they are both nuclear stains???
KERNECHTROTT (NUCLEAR FAST RED) COUNTERSTAIN
Kernechtrott is an anthraquinone dye. When complexed with aluminium,
it has excellent light fastness and does not require differentiation.
Bring 100ml of 5% aqueous aluminium sulphate to the boil. Remove from
heat and add 0.05g Kernechtrott (calcium red, nuclear fast red CI60760).
Boil until no more colour change is noted (approximately 5 to 15 minutes).
1. Deparaffinise and hydrate slides
2. Stain in Kernechtrott 2min.
3. wash in water.
4. dehydrate, clear and mount.
Waldrop, F.S., Puchtler, H., (1977). Stain Techn., 52(4);237.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital Westmead,
Locked Bag 4001, Westmead, 2145
Tel: (02) 9845 3306
Fax: (02) 9845 3318
>>> <email@example.com> 9/February/2001 05:01am >>>
Does anyone out there is histoland have a protocol for nuclear fast red
paraffin? Also is there a protocol for hematoxylin and nuclear fast red
together(instead of H & E)?
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