micro chatter in GI bx
My problem: micro chatter in Gi biopsies
I need suggestions for improving processing for GI biopsies.
Here's is what we do already:
* Fixation: 10% formalin fixed (our pathologists hate Hollande's...so
that is not an option.)
* Processing: We have a separate biopsy only run, which averages 50
biopsy blocks a day.
we presently use a 4 hour run with Pro-Par and ProSoft no heat on a
Shandon XP. We have tried a 6 hour (same results as a 4 hour) and a two
hour run (underprocessed tissue). We have also tried a 4 hour run with
regular alcohols/xylene processing....same results as we presently get.
We change/rotate processor reagents weekly or bi-weekly depending on needs.
* Microtomy: We cut two levels. Blocks are placed on ice trays and soaked
between levels. We cut at 4 microns. Chatter is not grossly visible on
Colorado Springs, CO
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