methyl green counterstain that works

From:Gayle Callis <>

Go into Histonet archives and look for Bob Schoonhoven's methyl green
counterstain, it is gangbusters, made up in an acetate buffer, for IHC
work.  Works with immunogold, enhanced, and should work with DAB or
whatever chromogen combination with MG gives good contrast. 

Many pathologists like hematoxylin for the detail it provides (what they
are used to looking at) morphologically.  You can dilute progressive
hematoxylins with distilled water, but they tend to break down after this
happens, but it will give delicate hematoxylin staining if you are bothered
by heavy blues.

Gayle Callis
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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