Zenker fixative -Reply

From:Tony Henwood <AnthonyH@chw.edu.au>

Jill,

No. There is no pigment to see except for possibly dichromate. This often
seems to wash out during staining (but not always).

Tony

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> Jill Songer <jtsonger@vt.edu> 14/February/2001 06:23am >>>
OK- dumb question of the day. Back in the old days, when Zenker's
fixative 
was used,  the slides had to treated with sodium thiosulfate, iodine, 
alcohols, etc to remove any pigment or precipitate formed by the
mercuric 
chloride used. My question-is this step still required with Zenker's that 
has zinc instead of mercuric chloride?

Thanks

**************************************************************************
Jill Songer HT (ASCP)
Supervisor, Anatomic Pathology
Virginia Tech
Veterinary Medicine Teaching Hospital

GO HOKIES!!!!!!






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