Shakin' and Dippin'

From:"John C. Dennis" <dennijc@vetmed.auburn.edu>

Dear Histofolk

Can anyone explain why now and again we hear/read that blocking solution
should be shaken, not washed, from the tissue sections prior to
applying primary or secondary solutions?

Once I was a shaker but there was no gift in simplicity.  I got uneven and
patchy signal patterns.  Now I'm a dipper and no longer have those
difficulties.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849





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