Re: storage of frozen tissue

From:Barry Rittman <>

It is possible that your tissue is becoming dehydrated during storage. I
would recommend that if using OCT as the support medium , that you cover
the surface with a thin layer and immediately freeze.
Additionally, if you add some ice cubes to the storage box  this will
help in preventing excessive dehydration. Dehydration  is less a problem
with tissues stored in liquid nitrogen due to the slower rate of removal
of moisture but can still be a problem with long term storage.
An additional note is that if you are keeping the specimen blocks in the
cryostat for hours after cutting, you will also get a significant amount
of dehydration of  the tissue.

Renee Thacker wrote:

> I am having trouble sectioning frozen tissue that has previously been
> sectioned.  The tissue will section wonderfully the first time then
> when resectioned weeks/months later the sections rip, separate from
> the embedding media, and sections in general just look awful.
> So I was wondering how people are storing frozen tissue that is
> sectioned and then will need to be sectioned at a later date stored
> (liquid nitrogen, -80 freezer wrapped in foil, etc.?). Generally I'm
> working with fresh frozen unfixed tissue but some fixed tissue as
> well.
> Thanks any help would be greatly appreciated!
> Renee
> --
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Renee Hoyle-Thacker
> CIIT Centers for Health Research
> 6 Davis Dr.
> Research Triangle Park  NC  27709
> Voice: (919) 558-1322
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

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