Re: micro chatter in GI bx
|From:||Katri Tuomala <email@example.com>|
I don't think your processing is at fault. GI bopsies are fragile and soft,
they need very gentle and SLOW microtome strokes, also cut them thinner than
standard (?) 5 mu. I cut mine at 3 mu. Good luck! Katri.
Hamilton, Ontario, Canada
> My problem: micro chatter in Gi biopsies
> I need suggestions for improving processing for GI biopsies.
> Here's is what we do already:
> * Fixation: 10% formalin fixed (our pathologists hate Hollande's...so
> that is not an option.)
> * Processing: We have a separate biopsy only run, which averages 50
> biopsy blocks a day.
> we presently use a 4 hour run with Pro-Par and ProSoft no heat on a
> Shandon XP. We have tried a 6 hour (same results as a 4 hour) and a two
> hour run (underprocessed tissue). We have also tried a 4 hour run with
> regular alcohols/xylene processing....same results as we presently get.
> We change/rotate processor reagents weekly or bi-weekly depending on needs.
> * Microtomy: We cut two levels. Blocks are placed on ice trays and soaked
> between levels. We cut at 4 microns. Chatter is not grossly visible on
> the waterbath.
> Any suggestions?
> Barb Davies
> Memorial Hospital
> Colorado Springs, CO
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