Re: micro chatter in GI bx
I don't think it is a processing problem,I think it is a microtomy
problem. When trimming these, dont rough trim in big steps and cut a few
sections at 3-4 microns before taking a section. Good sharp blades are
required also and have everything tight on the microtome.
03:59 PM 2/13/01 -0700, you wrote:
>My problem: micro chatter in Gi biopsies
>I need suggestions for improving processing for GI biopsies.
>Here's is what we do already:
>* Fixation: 10% formalin fixed (our pathologists hate Hollande's...so
>that is not an option.)
>* Processing: We have a separate biopsy only run, which averages 50
>biopsy blocks a day.
> we presently use a 4 hour run with Pro-Par and ProSoft no heat on a
>Shandon XP. We have tried a 6 hour (same results as a 4 hour) and a two
>hour run (underprocessed tissue). We have also tried a 4 hour run with
>regular alcohols/xylene processing....same results as we presently get.
>We change/rotate processor reagents weekly or bi-weekly depending on needs.
>* Microtomy: We cut two levels. Blocks are placed on ice trays and soaked
>between levels. We cut at 4 microns. Chatter is not grossly visible on
>Colorado Springs, CO
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