Re: clip-on in situ hybridization chambers

From:Abizar Lakdawalla <>

you might check the web site for mj research (, they have some
pretty good products for in situ PCR that minimizes evaporation especially one
called self-seal reagent


Karin Pittman wrote:

> Dear Histonetters
> I realize I may have been unclear in describing our routines for in situ
> hybridizations, and the reason for my plea for help. We routinely use
> coverslips or even parafilm to keep the moisture in on the slides, but are
> working with very thin sections which are often destroyed during the
> process of removing the coverslips. We had bought a special chamber for in
> situ incubations (a Grant Boekel SM 30) to try to avoid using coverslips
> but, despite various forms of keeping the conditions stable throughout, the
> humidity high and even covering the cover to eliminate a temperature
> differential, what we get are large drops of condensation on the upper
> surface of the chamber which drip down again onto the slides (which have in
> the meantime dried out slightly) causing wonderful patterns of artifacts
> and no reliable results. Using the coverslips inside the chamber gets us
> back to step 1 again and loss of tissue.
> Then we recently noticed that Sigma produces "Probe-clip press-seal
> incubation chambers" (catalogue number Z35,947-5) which are 22 x 40 mm for
> clipping right onto the slide. They are in effect microchambers, covering
> but not touching the tissue. This in theory would be fine if they too dont
> dry out. They sell in packages of 25 and are re-useable. Now my original
> question: Does anyone have any similar experience and has anyone tried
> these microchambers?
> thanks for your help
> Karin
> Dr. Karin Pittman
> Assoc. Professor
> Dept. of Fisheries and Marine Biology
> University of Bergen
> 5020 Bergen
> Norway
> tel: 47 55 58 44 72 (direct)
> fax: 47 55 58 44 50
> email:

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