Re: clip-on in situ hybridization chambers
From: | Karin Pittman <karin.pittman@ifm.uib.no> |
Dear Histonetters
I realize I may have been unclear in describing our routines for in situ
hybridizations, and the reason for my plea for help. We routinely use
coverslips or even parafilm to keep the moisture in on the slides, but are
working with very thin sections which are often destroyed during the
process of removing the coverslips. We had bought a special chamber for in
situ incubations (a Grant Boekel SM 30) to try to avoid using coverslips
but, despite various forms of keeping the conditions stable throughout, the
humidity high and even covering the cover to eliminate a temperature
differential, what we get are large drops of condensation on the upper
surface of the chamber which drip down again onto the slides (which have in
the meantime dried out slightly) causing wonderful patterns of artifacts
and no reliable results. Using the coverslips inside the chamber gets us
back to step 1 again and loss of tissue.
Then we recently noticed that Sigma produces "Probe-clip press-seal
incubation chambers" (catalogue number Z35,947-5) which are 22 x 40 mm for
clipping right onto the slide. They are in effect microchambers, covering
but not touching the tissue. This in theory would be fine if they too dont
dry out. They sell in packages of 25 and are re-useable. Now my original
question: Does anyone have any similar experience and has anyone tried
these microchambers?
thanks for your help
Karin
Dr. Karin Pittman
Assoc. Professor
Dept. of Fisheries and Marine Biology
University of Bergen
5020 Bergen
Norway
tel: 47 55 58 44 72 (direct)
fax: 47 55 58 44 50
email: karin.pittman@ifm.uib.no
www.uib.no
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