Re: Xylene usage
|From:||Connie McManus <firstname.lastname@example.org>|
In my case, it's someone's beloved pet or farm animal. However, I think it
depends upon the volume of tissues processed that determines the frequency
solutions are changed. I assume ya'll are doing >100 blocks/day. I average
approx. 30 slides/day with a range between 0 (like today... no tissues processed
last nite) to a full basket (70ish-- like I had on Monday). I change my
solutions once a month. All staining solutions are changed entirely at the same
time. On a weekly basis, I remove the post-eosin alcohols and the first abs
ETOH in the hydration series on the stainer and add fresh hematoxylin and eosin
just before use. I have never had any problems with improperly processed
tissues or staining. I agree with the attitudes expressed in this thread...
little things make all the difference in the quality of our work. It's not
such a hard thing to do to change solutions, but it is soooo important!
"Morrison, Michelle" wrote:
> Hi all. I would have to agree with you Dave, on all aspects you mentioned.
> The end product (optimally processed and stained slides)is what the
> pathologist needs to render an accurate dx. I work in a small lab and here
> quality is as important as quantity, if not more so. I couldn't imagine
> letting the reagents on the V.I.P. go beyond a weeks run. Somewhere in all
> those tissue filled casettes is someone's loved one, maybe yours.
> -----Original Message-----
> From: David Taylor Manager [mailto:DTMan@KINGMOWER.COM.AU]
> Sent: Tuesday, February 06, 2001 9:33 PM
> To: Histonet (E-mail)
> Subject: Xylene usage
> Dear fellow microtomist's,
> If I, a member of my family, close friend, enemy or other was having a
> specimen processed on a tissue processor, I would expect fresh reagents. Do
> use an enclosed processor if the money is there, do rotate like reagent from
> from old to new, do buy a TP that makes this easy, (VIP, or my personel
> choice, Leica TP1050.)
> We at K&M, and I'm sure others, can tell on opening a retort lid, if the wax
> should have been changed yesterday, or on embedding if the fixation was not
> In pathology, we receive money from patient's for a service provided.
> Provide a good service, change reagents, it does make a difference to the
> end product.
> David Taylor
> Laboratory Manager
> Drs King & Mower
> Adelaide, Australia
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