Re: TRAP stain

From:DDittus787@aol.com


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Retreive slides in citrate buffer ---------high power 5min 30 sec
                                                      30% power 11 min 30 sec
                                                       cool 15 min-----rinse 
in di water 2x
                                                        incubate with primary 
for 32 min at 37 degrees.                                       Dilution of 
antibody is your call , we use 1:80.
                                                 dana

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>Retreive slides in citrate buffer ---------high power 5min 30 sec
<BR>                                                      30% power 11 min 30 sec
<BR>                                                       cool 15 min-----rinse 
<BR>in di water 2x
<BR>                                                        incubate with primary 
<BR>for 32 min at 37 degrees.                                       Dilution of 
<BR>antibody is your call , we use 1:80.
<BR>                                                 dana</FONT></HTML>

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