Re: Slide adhesives

From:Roger Moretz <stamptrain@yahoo.com>

Darren:

I know I'm getting in on this a little late, and maybe
you have gotten the answer, but this is my experience.
 First, why are you trying to dissolve the mma with
xylene?  The beauty of the methacrylates is that they
are water permeable, thus permitting the use of
standard stain techniques directly.  Second.  The
technique I have always used with methacrylate is to
use slight heating of the slide with section to dry
and adhere the section to the slide.  In that way, I
have very seldom had any problem with losing sections
during staining (and that includes a lot of special
stains.)  Hope this helps.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

--- Darren Robinson <drobinson@ciit.org> wrote:
> Does anybody out there have an idea as to how I can
> get large 
> methylmethacrylate sections (3"x3") to stick onto
> glass slides.  I am 
> cutting some really nice large sections, getting
> them onto the slides, then 
> losing them when I either try and dissolve the resin
> in xylene or stain 
> them with heamalum.  I've tried APES, and sta-on,
> and am running out of 
> ideas fast.
> 
> Many thanks,
> 
> Darren
> 
> ****************************************
> Darren Robinson
> Postdoctoral Fellow
> CIIT Centers for Health Research
> 6 Davis drive
> Research Triangle Park
> North Carolina
> 27709
> 
> Tel. 919-5581385
> Email. DRobinson@ciit.org
> ****************************************
> 
> 


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