Re: Searching (Paul Klosen)

>I search for an aqueous mounting medium (slides are stained with AEC)!!

Try Crystalmount. You cover the stained hydrated sections with it and let
it dry. It forms a protective film over the sections, which can then be
coverslipped with toluene-based media like Eukitt or DPX.

While Cryalmount dries, its refractive index increases to values close to
that of glass, thus you end up with a very nicely transparent preparation
comparable to dehydrated and toluene-cleared sections.

Crystalmount is sold by Biomeda if I remember well. Shandon has a similar
product, Aquaperm if I remember correctly. Shandon's Immunomount would do
also, but it is used like a conventional mounting medium (to apply the
coverslip) and it's refractive index is nowhere near that of glass, thus
the preparations are not as transparent as with the Crystalmount or the


                         (o -) O
Paul Klosen, PhD
CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
Universite Louis Pasteur
12 rue de l'Universite
F-67000 Strasbourg, FRANCE
tel:    fax:

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