<html>
Monica,<br>
I use a modified version of Roque's
Chromotrope 2R<br>
<br>
<b>Chromotrope
2R
pH solution to
2.0-2.2
<br>
</b>
Chromotrope2R
2.0gm<br>
Aniline
Blue
0.5gm<br>
Phosphotungstic
acid
1.0gm<br>
Hydrochloric acid,0.02N
solution 100cc<br>
<br>
1 Deparaffinize and hydrate to distilled water.<br>
2.Stain in Weigert's Iron Hematoxylin for 10 minutes<br>
3.Wash in tap water for 10 minutes, rinse in distilled water<br>
4. 1% Phosphomolybdic acid(aqueous) for 1-<u>3</u> minutes<br>
5 Rinse well in distilled water<br>
6. Stain in Chromotrope aniline blue solution for 8 mminutttes<br>
7 Rinse in distilled water, dehydrate through graded alcohols,clear in
xylene, coverslip<br>
<br>
RESULTS<br>
Hyaline
bodies Bright
blue or red with blue periphery<br>
Chromatin stains pink or
bluish<br>
Nucleoli
red<br>
Cytoplasm
pink to purplish<br>
collagen and reticulin blue<br>
<br>
the following is a note from Roque's paper<br>
These variations in staining depend on the structure of the hyaline
bodies, which consist of a core of basic proteins surrounded by an outer
layer of glycoproteins.<br>
<br>
Reference Laboratory Investigation, vol 2,NO.1,1953<br>
<br>
Rena Fail AS HT(ASCP)<br>
SS/IHC LAB<br>
Medical University of SC<br>
Charleston, SC<br>
<br>
Hope <b>my</b> spell checker workeed <b>:)<br>
<br>
</b>At 10:08 PM 2/14/01 +0930, you wrote:<br>
<blockquote type=cite class=cite cite>Can someone provide me with method
for Chromatotrope-Aniline Blue for<br>
colagen and mallory bodies please?<br>
<br>
Regards,<br>
<br>
Monika Stanczew<br>
Histology<br>
Royal Darwin Hospital,<br>
Australia<br>
e-mail: Monika.Stanczew@bigpond.com</blockquote><br>
</html>