Re: Frozen section adherence again!!!!!!!
|From:||Shirley Powell <powell_sa@Mercer.EDU>|
I have had experience with sections washing from positive slides when post fixed
in B5 or a mercury fixative. Not being an expert in chemistry, I can only
speculate as to the reason, so I will not bore you with my thoughts. But my
remedy is to float the tissue sections on a water bath with .025% agar
(10mL/1000 deionized H20 ), pick up sections on plain slides, drain the slides
well and place on a flat warming stage at 60 degrees for an hour, or for brain
or bone, overnight. The sections survive HIER and immunostaining on the
"Weems, Joyce" wrote:
> Oops - we didn't mean to ignore you!! I'm not the expert here, but have you
> tried charged slides? They seem to be the answer to all our tissue adherence
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
> -----Original Message-----
> From: Szigeti Csaba [SMTP:firstname.lastname@example.org]
> Sent: Friday, February 09, 2001 5:06 AM
> To: email@example.com
> Subject: Frozen section adherence again!!!!!!!
> Please write me some tips....
> my spinal cord slices goes down from the silan slides during the
> of in situ hibr.
> I wrote some days ago but not any answer yet......
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