I have had experience with sections washing from positive slides when post fixed
in B5 or a mercury fixative.  Not being an expert in chemistry, I can only
speculate as to the reason, so I will not bore you with my thoughts.  But my
remedy is to float the tissue sections on a water bath with .025% agar
(10mL/1000 deionized H20 ), pick up sections on plain slides, drain the slides
well and place on a flat warming stage at 60 degrees for an hour, or for brain
or bone, overnight.  The sections survive HIER and immunostaining on the
Ventana.

"Weems, Joyce" wrote:

> Oops - we didn't mean to ignore you!! I'm not the expert here, but have you
> tried charged slides? They seem to be the answer to all our tissue adherence
> problems.
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
>
>         -----Original Message-----
>         From:   Szigeti Csaba [SMTP:szigcs@bio.u-szeged.hu]
>         Sent:   Friday, February 09, 2001 5:06 AM
>         To:     histonet@pathology.swmed.edu
>         Subject:        Frozen section adherence again!!!!!!!
>
>         Please write me  some tips....
>         my spinal cord slices goes down from the silan slides during the
> protokoll
>         of in situ hibr.
>         I wrote some days ago but not any answer  yet......
>         Csaba
>
>