Date sent:      	Fri, 09 Feb 2001 11:06:15 +0100
From:           	Szigeti Csaba <szigcs@bio.u-szeged.hu>
Subject:        	Frozen section adherence again!!!!!!!
To:             	histonet@pathology.swmed.edu

> Please write me  some tips....
> my spinal cord slices goes down from the silan slides during the protokoll
> of in situ hibr.
Csaba, some of the procedures for in situ are not conducive for 
keeping sections on the slide (especially tissue from central and 
peripheral nervous system). I use commercially prepared "charged" 
slides whether the tissue is fixed or unfixed. Allow the sections to 
air dry thoroughly before you begin your procedure (fixed tissue) or 
before you fix (if possible). If you provide some more information 
about your procedure (e.g. fixation of tissue, type of probe, 
pre-treatments etc. ) then I am sure someone will be able to help 
solve your problem.


Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360