Re: Fat Stains

From:
TWARE4U@aol.com

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Dear Judi,
Lipids/fat stains are usually performed on frozen sections of tissues fixed 
in neutral buffered formalin.  As you already know  fats are soluble in ethyl 
alcohol so you would not be able to process the specimen first. Triglycerides 
stain best with the Oil Red O stain and the Cholesterol  will stain with  
Schultz reaction. Both of these stains are found in the AFIP  Laboratory 
Methods in Histotechnology (red cover).
I hope this is helpful.
Lisa Wierzbicki HT/MLT (ASCP)

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>Dear Judi,
<BR>Lipids/fat stains are usually performed on frozen sections of tissues fixed 
<BR>in neutral buffered formalin.  As you already know  fats are soluble in ethyl 
<BR>alcohol so you would not be able to process the specimen first. Triglycerides 
<BR>stain best with the Oil Red O stain and the Cholesterol  will stain with  
<BR>Schultz reaction. Both of these stains are found in the AFIP  Laboratory 
<BR>Methods in Histotechnology (red cover).
<BR>I hope this is helpful.
<BR>Lisa Wierzbicki HT/MLT (ASCP)</FONT></HTML>

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