Re: Daily Digest
| From: | greg tesdall <gtesdall@yahoo.com> |
I have had the Sukura since 1985 and use it for
histo/cyto. I have never had a coverslip come off. For
us it's a great machine. >
----------------------------------------------------------------------
>
> Date: 11 Feb 2001 03:58:01 -0600
> From: RichardWHorobin@aol.com
> Subject: Masson on resin section
>
>
>
> Diane Berenek wrote saying:
>
> > I need a procedure for staining Methacrylate
> sections using Masson
> > Trichrome. Would it be the same for Paraffin
> embedded sections, or is there
> > some modification needed?
>
> By methacrylate do you mean the 'water miscible'
> glycol methacrylate (GMA),
> or methyl methacrylate (MMA)?
>
> If MMA, since the resin is removed prior to
> staining, there should be no
> particular problem using a 'designed for paraffin'
> procedure. I will rephrase
> that: no EXTRA problems, as Masson's trichrome is an
> innately complex
> procedure.
>
> If GMA, the resin is not (cannot) be removed prior
> to staining, and this
> greatly influences the staining process. Stains
> involving sizeable dyes -
> such as aniline blue or light green - will tend to
> understain their usual (ie
> 'paraffin section usual') targets, but to give
> background resin staining
> which is hard to remove (try alcohol). The acidic
> phosphomolybdic acid
> exacerbates this effect. If you find a way around
> these problems, do please
> let me know! The simpler trichromes, such as van
> Gieson's, can be done on GMA
> material, especially if penetration aids such as
> alcohol are used to swell
> the resin.
>
> Richard Horobin
> Institute of Biomedical & Life Sciences, University
> of Glasgow
> T direct +44-1796-474 480 --- E
> RichardWHorobin@aol.com
> "What should we expect? Everything."
>
>
>
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> contained in the following MIME Information.
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> <HTML><FONT FACE=arial,helvetica><FONT SIZE=2>
> <BR>Diane Berenek wrote saying:
> <BR>
> <BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT:
> #0000ff 2px solid; MARGIN-LEFT:
> 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">I need a
> procedure for staining
> Methacrylate sections using Masson
> <BR>Trichrome. Would it be the same for Paraffin
> embedded sections, or is
> there
> <BR>some modification needed?</FONT><FONT
> COLOR="#000000" SIZE=3
> FAMILY="SANSSERIF" FACE="Arial"
> LANG="0"></BLOCKQUOTE>
> <BR></FONT><FONT COLOR="#000000" SIZE=2
> FAMILY="SANSSERIF" FACE="Arial"
> LANG="0">
> <BR>By methacrylate do you mean the 'water miscible'
> glycol methacrylate
> (GMA),
> <BR>or methyl methacrylate (MMA)?
> <BR>
> <BR>If MMA, since the resin is removed prior to
> staining, there should be no
> <BR>particular problem using a 'designed for
> paraffin' procedure. I will
> rephrase
> <BR>that: no EXTRA problems, as Masson's trichrome
> is an innately complex
> <BR>procedure.
> <BR>
> <BR>If GMA, the resin is not (cannot) be removed
> prior to staining, and this
> <BR>greatly influences the staining process. Stains
> involving sizeable dyes -
> <BR>such as aniline blue or light green - will tend
> to understain their usual
> (ie
> <BR>'paraffin section usual') targets, but to give
> background resin staining
> <BR>which is hard to remove (try alcohol). The
> acidic phosphomolybdic acid
> <BR>exacerbates this effect. If you find a way
> around these problems, do
> please
> <BR>let me know! The simpler trichromes, such as van
> Gieson's, can be done on
> GMA
> <BR>material, especially if penetration aids such as
> alcohol are used to swell
>
> <BR>the resin.
> <BR>
> <BR>Richard Horobin
> <BR>Institute of Biomedical & Life Sciences,
> University of Glasgow
> <BR><B>T direct +44-1796-474 480 --- E
> RichardWHorobin@aol.com</B>
> <BR><I>"What should we expect?
> Everything."</I></FONT></HTML>
>
> - --part1_db.101ac806.27b7961f_boundary--
>
>
>
----------------------------------------------------------------------
>
> Date: 11 Feb 2001 14:45:54 -0600
> From: "Bill Sinai" <bills@icpmr.wsahs.nsw.gov.au>
> Subject: Re: Daily Digest
>
>
> Dear All,
> We have had the Sakura Tape Coverslipper for about
> 10 years now and have
> approximately 2,000,000 slides which the coverslip
> and tissue have come
> away. This can begin happening as soon as 2 years
> after coverslipping but
> most seem to last at least 4-5 years.
>
> We sent some slides to Sakura in Japan and their
> suggestion was to place the
> coverslip in xylene and re-attach it to the slide.
> Our Director decided we
> should return to glass coverslipping with an
> appropriate mountant to try to
> preserve the slides for the 20 years we are required
> to keep them.
>
> Bill Sinai
> Laboratory Manager
> Tissue Pathology ICPMR
> P.O. Box 533
> Wentworthville NSW Australia 2145
>
>
############################################################################
> ###############
>
> Subject: Re: Daily Digest
>
>
> > We have had the Tissue Tek coverslipper now for
> about 4 years. I have
> > looked at the slide we first coverslipped with it
> and have seen no
> > problems. We did do some environmental changes to
> make sure that the
> > manufactures suggestions on storage were met
> (temperature and humidity).
> > I think this is very important.
> >
> > Rae Ann Staskiewicz HT(ASCP)
> > Galesburg Animal Disease Lab
> > Galesburg, IL
> >
> > Mrhabbs@aol.com wrote:
> > >
> > > My lab is looking at the Sakura Tissue Tek
> automatic coverslipper
> (tape).
> > > Could I please get some feedback on the long
> term quality of sections
> and any
> > > other comments that people who have experience
> with
=== message truncated ===
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