Re: Cromogen/Substrate IHC question

From:Victoria Baker <>

<P>Hi,</P> <P>When switching detection systems it is always safer to check your dilutions again and do a titration run.  But instead of switching this, why not get the red substrate that Vector has for Alk-Phos?  The kit is like $65.00 and will save more in time an effort on your part.  I keep both systems in my lab (HRP & Alk Phos) with red chromogen.  We've had good results for the most part.</P> <P>Good luck.</P> <P>Vikki Baker</P> <ADDRESS>American Health Foundation</ADDRESS> <ADDRESS>Valhalla, New York 10595<BR></ADDRESS> <P>  <B><I>"Bennett, Catherine (Katie)" <></I></B> wrote: <BR> <BLOCKQUOTE style="BORDER-LEFT: #1010ff 2px solid; MARGIN-LEFT: 5px; PADDING-LEFT: 5px">Advice needed:<BR><BR>If I switch an IHC procedure over from an ABC-HRP kit to an ABC-Alk Phos<BR>kit, I shouldn't have to adjust my primary and secondary antibody<BR>concentrations, right? (I want to change cromogen labels from DAB to<BR>Vector-Red.)<BR><BR>TIA.<BR><BR>*********************************<BR>Catherine "Katie" Bresee Bennett<BR>Sr. Research Technologist<BR>Lovelace Respiratory Research Institute<BR>Albuquerque, New Mexico<BR><BR></BLOCKQUOTE><p><br><hr size=1><b>Do You Yahoo!?</b><br> <a href=>Yahoo! Auctions</a> - Buy the things you want at great prices.
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