Re:Counterstain for paraffin embedded sections
From: | Liv =?iso-8859-1?Q?Gr=F6ntoft?= <LivGr@IHM.LiU.SE> |
Hi Kathie
My experience is the same as yours. Methyl Green stains very weakly generaly.
Why dont´t you try Light Green. It works exellent when you use DAB as
chromogen.With my protocol you stain 45 seconds, rinse in 95%, 99%, Xylen.
Light GreenSF(C.I.42095) 0.2g,100ml distilled water,Acetic Acid 100% 0.2ml.
Dilute 1:5
Good luck
Liv
Liv Gröntoft
Rättsmedicinska Avdelningen
Universitetssjukhuset
581 85 Linköping
Sweden
Phone +46 13 36 42 58
Fax +46 13 36 42 70
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