Re: Counterstain for Paraffin embedded sections??
|From:||Shirley Powell <powell_sa@Mercer.EDU>|
When nuclear antigens are stained in my lab, I use light green stain used in
silver stains, but rinses should be done with tap water after staining because
deionized water will wash it out of the sections.
"Dr. Allen A. Smith" wrote:
> Methyl green is a very fussy stain. It is adversely affected by many other
> Kernechtrot, hematoxylin, brazalum, and gallocyanin are versatile nuclear
> stains. (Formulas are available in Kiernan's HISTOLOGICAL AND HISTOCHEMICAL
> METHODS.) Kernechtrot usually requires no modification to fit into your
> Allen A. Smith, Ph.D.
> Barry University School of Graduate Medical Sciences
> Podiatric Medicine and Surgery
> Miami Shores, Florida
> ----- Original Message -----
> From: Kathie Berghorn <firstname.lastname@example.org>
> To: HistoNet Server <email@example.com>
> Sent: Thursday, February 08, 2001 5:49 PM
> Subject: Counterstain for Paraffin embedded sections??
> > Hi.
> > I am hoping someone can help us. We just recently began
> > working with paraffin embedded sections. We are getting good
> > staining with the primary antibodies we are using. The problem is
> > the counterstain. We would like to use methyl green, but we cannot
> > get the tissue to take it up despite our best efforts. We left it on
> > for an hour, we made up new methyl green, all to no avail. We are
> > using DAB as the chromogen for our primary antibody staining. Could
> > someone recommend a good counterstain that would provide nice
> > contrast and work in paraffin sections?
> > Any recommendations would be most appreciated! Thanks.
> > Kathie
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