Methyl green is a very fussy stain.  It is adversely affected by many other
stains.
Kernechtrot, hematoxylin, brazalum, and gallocyanin are versatile nuclear
stains. (Formulas are available in Kiernan's HISTOLOGICAL AND HISTOCHEMICAL
METHODS.)  Kernechtrot usually requires no modification to fit into your
protocol.

Allen A. Smith, Ph.D.
Barry University School of  Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida

----- Original Message -----
From: Kathie Berghorn <kab35@cornell.edu>
To: HistoNet Server <histonet@pathology.swmed.edu>
Sent: Thursday, February 08, 2001 5:49 PM
Subject: Counterstain for Paraffin embedded sections??


> Hi.
> I am hoping someone can help us.   We just recently began
> working with paraffin embedded sections.  We are getting good
> staining with the primary antibodies we are using.  The problem is
> the counterstain.  We would like to use methyl green, but we cannot
> get the tissue to take it up despite our best efforts. We left it on
> for an hour, we made up new methyl green, all to no avail.  We are
> using DAB as the chromogen for our primary antibody staining.  Could
> someone recommend a good counterstain that would provide nice
> contrast and work in paraffin sections?
>
> Any recommendations would be most appreciated!  Thanks.
>
> Kathie
>
>