Re: Alcian Blue
| From: | RichardWHorobin@aol.com |
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Sharyn asks:
> Does anyone have a source for alcian blue dye? All
> our vendors seem to be out and are not forcasting any
> date of re-stocking.
HISTORICAL ASIDE - This 'alcian blue drought' has been a recuring phenomenon
for more than 20 years. A shortage - - and then more dye appears. Where does
it come from? Dunno.
>
Yes! PRACTICAL SUGGESTION - Charles Churukian, well known to many of you I am
sure, did some work using a commercially available AB alternative several
years ago. Recently he extended this, see below. And as you see I am not a
neutral party:
Alcian blue pyridine variant - A superior alternative to alcian blue 8GX:
staining performance and stability.
Churukian CJ, Frank M & Horobin RW. 2000. Biotechnic & Histochemistry. 75:
147-150.
COMMENT 1 - Its 'superior' because it stains exactly as does AB but the
powder and the solutions are more stable
COMMENT 2 - If youre after reprints try Matt Frank, I havent any. He is at
the Biological Stain Commision Lab in Rochester. His email address is:
<Mathew_Frank@URMC.Rochester.edu>
Bye - Richard Horobin
Institute of Biomedical & Life Sciences, University of Glasgow
T direct 01796-474 480 --- E RichardWHorobin@aol.com
"What should we expect? Everything."
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Sharyn asks:
<BR>
<BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">Does anyone have a source for alcian blue dye? All
<BR>our vendors seem to be out and are not forcasting any
<BR>date of re-stocking. </FONT><FONT COLOR="#000000" SIZE=3 FAMILY="SANSSERIF" FACE="Arial" LANG="0"></BLOCKQUOTE>
<BR>
<BR>HISTORICAL ASIDE - This 'alcian blue drought' has been a recuring </FONT><FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0">phenomenon </FONT><FONT COLOR="#000000" SIZE=3 FAMILY="SANSSERIF" FACE="Arial" LANG="0">
<BR>for more than 20 years. A shortage - - and then more dye appears. Where does
<BR>it come from? Dunno.
<BR></FONT><FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0">
<BR>Is anyone using a <BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">good substitue.</BLOCKQUOTE>
<BR>
<BR>Yes! PRACTICAL SUGGESTION - Charles Churukian, well known to many of you I am
<BR>sure, did some work using a commercially available AB alternative several
<BR>years ago. Recently he extended this, see below. And as you see I am not a
<BR>neutral party:
<BR>
<BR>Alcian blue pyridine variant - A superior alternative to alcian blue 8GX:
<BR>staining performance and stability.
<BR>Churukian CJ, Frank M & Horobin RW. 2000. Biotechnic & Histochemistry. 75:
<BR>147-150.
<BR>
<BR>COMMENT 1 - Its 'superior' because it stains exactly as does AB but the
<BR>powder and the solutions are more stable
<BR>COMMENT 2 - If youre after reprints try Matt Frank, I havent any. He is at
<BR>the Biological Stain Commision Lab in Rochester. His email address is:
<BR> <Mathew_Frank@URMC.Rochester.edu>
<BR>
<BR>Bye - Richard Horobin
<BR>
<BR>Institute of Biomedical & Life Sciences, University of Glasgow
<BR><B>T direct 01796-474 480 --- E RichardWHorobin@aol.com</B>
<BR><I>"What should we expect? Everything."</I></FONT></HTML>
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