RE: Daily Digest

From:"Schelling, Scott" <>

I would like to unsubscribe to the daily digest.  Thanks much for honoring
my request.  

-----Original Message-----
From: HistoNet Server []
Sent: Monday, February 05, 2001 12:00 AM
To: HistoNet Server
Subject: Daily Digest


Date: 4 Feb 2001 16:30:23 -0600
Subject: on job trainee

I would like to know if anyone is offering on job daughter is 
interested in becoming a histotechnician.i have been a histotech for 8.5 
yrs. however i cannot train her at my facility.we live in oklahoma north of 
oklahoma city.we are willing to move to a location,so she could begin ojt 
anyone  out there let me know. i know it is still possible before the year 
2005 .  thank you  Histojoe. P.S. you may reply or e-mail me at
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Date: 4 Feb 2001 17:15:31 -0600
Subject: Region VII S/C in Colorado

Contact John McGinley to register

Location:  Children's Hospital  Denver, Colorado

Colorado Society for Histotechnology and NSH Region VII S/C
Session 1- Friday April 20, 2001
#1a Immunohistochemistry Update - 2001"
Richard Cartun, PhD Hartford Hospital, CT
Case studies will be used to review current applications of IHC to solid
tumor identifications, prognostic markers in breast cancer, cytology, and
infectious disease detection.  Use of molecular pathology methods will also
be discussed. 

#1b "The Animal & Human Prion Diseases  (AKA The TSE'S)"
Elizabeth S. Williams DVM, PhD, Diplomat ACVP
University of Wyoming
This presentation will review the human animal transmissible spongiform
encephalopathies (CJD, VCJD, BSE, CWD, TME, etc.); what they are and how we
diagnose them.

#1c "The Chemistry of Tissue Processing"
Jerry Fredenburgh PhD,  Richard Allen 
This is a workshop which consists of a discussion of the basic chemistry and
chemical reactions that take place during tissue processing.  Also discussed
are how these chemical reactions influence the results of processing and
staining. A manual and slides will be used as teaching aids. 

#1d "What the Heck is HIPAA and Why Should I Care?"
Jackie Driscoll, MT(ASCP)SM,  Patient Choice
Gain a general understanding of the three main areas of focus of the new
federal regulations: electronic transaction, security and privacy. Learn how
the regulations may affect your work environment and your rights as a
private citizen.
Session 2- Saturday April 21, 2001 AM

#2a "Are You Singing The Blues?"
Gayle Callis, BS, MT, HT, HTL(ASCP) 
Montana State University
This is a hands on hematoxylin and eosin staining workshop using several
commercially made hematoxylin formulations.  The first half will be a brief
discussion of the history. Formulations and chemistry of H&E staining will
be followed by a hands-on portion.  If time allows troubleshooting hints on
problems commonly encountered with the H&E will be presented.
Troubleshooting hints are part of the handout for future reference.  NBF
fixed tissue sections will be supplied but attendees may wish to stain
sections from their labs, a minimum of four slides, even though there are
seven different hematoxylins available for staining.
#2b  "Application of Basic IHC Including Incorporating new Antibodies in
Your Lab"
Denise Sapier, HT, BS   Dako Corporation
This workshop will focus on the basics of Immunohistochemistry to include
discussion on the selection of monoclonal versus polyclonal antibodies in
routine work, and troubleshooting hints on retrieval pretreatment and
background staining.  Participants will also learn how to incorporate a new
antibody or a new procedure into their laboratory routine.

#2c  "Applications of Immunohistochemistry to Diagnostic Hematopathology"
Richard Cartun, PhD Hartford Hospital, CT
This lecture will review current applications of IHC to diagnostic
hematopathology.  Case studies will be used to demonstrate the use of
antibodies to CD5, CD10, CD23, ALK-1, Bcl-6, CyclinD1 and others.  Tissue
pretreatments and detection systems will be discussed.

Session 3- Saturday April 21, 2001 PM		
#3a "The Her-2/neu Gene and Protein in Breast Cancer: Prognostic, Predictive
and Target of Therapy."
Robert Gasparini, MS, CLSp(CG) Ventana
Over the last 18 months, the question in the testing labs has changed from
"should we test for Her2?" to "How should we test for Her2?" Data on the two
most common methods of testing, IHC and FISH will be discussed in detail.
The Her-2/neu oncogene represents the next generation of "molecular markers"
that have the potential to be utilized for: a) prognostic factor; b)
predictive factor; and c) target of new therapies.

#3b " Organs and the Role of  Fetal Stem Cells " 
Gail Deutsch, MD   Children's Hospital, Denver
There has been tremendous strides in stem cell research in the past couple
of years.  Find out how distinctly different organs emerge from
multipotential precursor cell populations in the developing murine embryo.  

#3c "Tricks of the Trade" moderated by: Donna Harp, HT(ASCP) Ventana
Bring your problems and bring your solutions in this round table format.
Participants will share their experiences, thoughts and opinions on various
technical issues.


Date: 4 Feb 2001 19:30:47 -0600
From: "Paula Wilder" <>
Subject: QC procedures

Hello Everyone!

I need to write up quality control procedures in pathology, especially for 
accessioning.  My manager also has required me to include things like - how 
many errors before a verbal warning, how many errors before a written 
warning.  Does anyone know where I might at least obtain some information on

this?  I have been asking around, but have not come up with anything 
concrete.  Any help would be immensely appreciated.  We do 18,000 cases a 

Paula Wilder
St. Joseph Medical Center
Towson, MD.
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Date: 4 Feb 2001 19:31:01 -0600
From: "Paula Wilder" <>
Subject: Cryostat backup

Hello Everyone!

What does anyone do when a back-up cryostat fails? I am a little concerned 
about our frozen area.  Our primary cryostat has been out for major repairs 
and the back-up is very old.  Thanks for any suggestions!  (And no- we 
cannot purchase a new one!)

Paula Wilder
St. Joseph Medical Center
Towson, MD.
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