Hello.  We have done this using DAB and the Ventana ES stainer.  Prepare
your H&E then do you IHC.  No counterstain needed with the IHC.

Tom Mc Nemar, HT(ASCP)
Pathology Supervisor
Licking Memorial Hospital
Newark, Ohio

> -----Original Message-----
> From:	C.M. vander Loos [SMTP:c.m.vanderloos@amc.uva.nl]
> Sent:	Wednesday, January 31, 2001 9:58 AM
> To:	Michelle Peiffer
> Cc:	histonet@pathology.swmed.edu
> Subject:	Re: DAB and H&E counterstain
> 
> >Hi all,
> >
> >We have a researcher requesting IHC and H&E on the same slide (trying to
> >save money).  We are using DAB, then trying to counterstain with H&E, and
> >as expected the DAB is drowned out by the eosin.  Could anybody share a
> >method of doing this?  Would adding Nickle to the DAB help?  Thanks in
> >advance for all your good advice.
> >
> >
> >Michelle Peiffer
> >*************************************************************
> >Electron Microscope Facility for the Life Sciences
> >Penn State University Biotechnology Institute
> >001 South Frear Lab
> >University Park PA 16802
> >
> >phone: 814-865-0212
> >email:  mlk101@psu.edu
> >**************************************************************
> >
> 
> *******
> Hi Michelle,
> 
> Even at this stage you could save your specimens!!
> The DAB precipitate can be "osmified" turning it into black. I haven't any
> ideas about concentration or incubation time, I just remembered this
> method
> from a long time ago that it really worked. Since you work at an EM
> facility this osmification can't be very much of a problem!
> 
> Chris van der Loos
> Amsterdam, The Nethelands
> 
>