Jim,

Caspase 3, like the other caspases exists in cells as a inactive proenzyme.
Cleavage (activation) of caspase 3 is the indicator for apoptosis. If
apoptosis is what you are looking at, then check out Promegas new
anti-active Caspase 3 antibody directed against the p18 cleavage fragemtn
(active form) of Caspase.

Brett


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly@merck.com
   

> ----------
> From: 	Jim Manavis[SMTP:jim.manavis@imvs.sa.gov.au]
> Sent: 	Tuesday, February 13, 2001 12:56 AM
> To: 	Histonet
> Subject: 	Caspase 3
> 
> What antibodies and retrievals are there being used for Caspase 3.
> 
> Thanks
> 
> Jim Manavis
> Senior Hospital Scientist
> Department of Neuropathology
> Institute of Medical & Veterinary Science
> Adelaide, SA, 5000
> Australia
> Phone: 61-08-8222-3668
> FAX: 61-08-8222 3204
> e.mail: jim.manavis@imvs.sa.gov.au
> Disclaimer: Not this little black duck!
> 
> 
>