Osteocyte Cell Viability

From:kalschev@svm.vetmed.wisc.edu

     
     John Baker,
     
     H & E staining will identify cell morphology and viability to some 
     degree although compromised by the decal process. The area of research 
     using an assay stain to differentiate live cells from dead cells by 
     labeling and determining metabolic activity of the cellular function 
     is in the literature. It does require access to a confocal laser 
     microscope with special filters.  We are working with fresh, unfixed 
     cartilage. Consider checking some of the recent Arthroscopy and Bone 
     Journals. 
     
     
     
     Vicki Kalscheur
     School of Veterinary Medicine
     2015 Linden Drive West
     University of WI - Madison, WI  53706-1102
     
     (608) 262-8534     Fax (608) 263-7930      
     
     kalschev@svm.vetmed.wisc.edu




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