Masson on resin section
| From: | RichardWHorobin@aol.com |
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Diane Berenek wrote saying:
> I need a procedure for staining Methacrylate sections using Masson
> Trichrome. Would it be the same for Paraffin embedded sections, or is there
> some modification needed?
By methacrylate do you mean the 'water miscible' glycol methacrylate (GMA),
or methyl methacrylate (MMA)?
If MMA, since the resin is removed prior to staining, there should be no
particular problem using a 'designed for paraffin' procedure. I will rephrase
that: no EXTRA problems, as Masson's trichrome is an innately complex
procedure.
If GMA, the resin is not (cannot) be removed prior to staining, and this
greatly influences the staining process. Stains involving sizeable dyes -
such as aniline blue or light green - will tend to understain their usual (ie
'paraffin section usual') targets, but to give background resin staining
which is hard to remove (try alcohol). The acidic phosphomolybdic acid
exacerbates this effect. If you find a way around these problems, do please
let me know! The simpler trichromes, such as van Gieson's, can be done on GMA
material, especially if penetration aids such as alcohol are used to swell
the resin.
Richard Horobin
Institute of Biomedical & Life Sciences, University of Glasgow
T direct +44-1796-474 480 --- E RichardWHorobin@aol.com
"What should we expect? Everything."
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>
<BR>Diane Berenek wrote saying:
<BR>
<BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">I need a procedure for staining Methacrylate sections using Masson
<BR>Trichrome. Would it be the same for Paraffin embedded sections, or is there
<BR>some modification needed?</FONT><FONT COLOR="#000000" SIZE=3 FAMILY="SANSSERIF" FACE="Arial" LANG="0"></BLOCKQUOTE>
<BR></FONT><FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0">
<BR>By methacrylate do you mean the 'water miscible' glycol methacrylate (GMA),
<BR>or methyl methacrylate (MMA)?
<BR>
<BR>If MMA, since the resin is removed prior to staining, there should be no
<BR>particular problem using a 'designed for paraffin' procedure. I will rephrase
<BR>that: no EXTRA problems, as Masson's trichrome is an innately complex
<BR>procedure.
<BR>
<BR>If GMA, the resin is not (cannot) be removed prior to staining, and this
<BR>greatly influences the staining process. Stains involving sizeable dyes -
<BR>such as aniline blue or light green - will tend to understain their usual (ie
<BR>'paraffin section usual') targets, but to give background resin staining
<BR>which is hard to remove (try alcohol). The acidic phosphomolybdic acid
<BR>exacerbates this effect. If you find a way around these problems, do please
<BR>let me know! The simpler trichromes, such as van Gieson's, can be done on GMA
<BR>material, especially if penetration aids such as alcohol are used to swell
<BR>the resin.
<BR>
<BR>Richard Horobin
<BR>Institute of Biomedical & Life Sciences, University of Glasgow
<BR><B>T direct +44-1796-474 480 --- E RichardWHorobin@aol.com</B>
<BR><I>"What should we expect? Everything."</I></FONT></HTML>
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