Immunohistochemistry Puzzler (Hermina Borgerink)

Hi Elizabeth,

Just another thought in regard to the problem you are having with
hydrogen peroxide blocking prior to your primary antibody incubation.
To avoid this, you might try quenching for endogenous peroxidases after
your primary antibody incubation, rather than prior to it.  Some people
even perform this step after the secondary antibody incubation, and it
is preferred to do it this way when staining frozen sections. Good luck!


Hermina Borgerink, BA, HT(ASCP)IHQ
Department of Pathology
Wake Forest University School of Medicine
Winston-Salem, N.C. 27157
PH (336) 716-1538
Fax (336) 716-1515

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