Cromogen/Substrate IHC question

From:"Bennett, Catherine (Katie)" <>

Advice needed:

If I switch an IHC procedure over from an ABC-HRP kit to an ABC-Alk Phos
kit, I shouldn't have to adjust my primary and secondary antibody
concentrations, right?  (I want to change cromogen labels from DAB to


Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico

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