Cromogen/Substrate IHC question

From:"Bennett, Catherine (Katie)" <cbennett@lrri.org>

Advice needed:

If I switch an IHC procedure over from an ABC-HRP kit to an ABC-Alk Phos
kit, I shouldn't have to adjust my primary and secondary antibody
concentrations, right?  (I want to change cromogen labels from DAB to
Vector-Red.)

TIA.

*********************************
Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico




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