Counterstain for Paraffin embedded sections?? -Reply
|From:||Tony Henwood <AnthonyH@chw.edu.au>|
Check the pH of the Methyl Green (should be around 4). Also keep the
water and alcohol rinses short. Maybe dehydrating in n-butanol might
You could try a haematoxylin counterstain (eg Harris's, Mayers).
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital Westmead,
Locked Bag 4001, Westmead, Australia, 2145.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
>>> Kathie Berghorn <firstname.lastname@example.org> 9/February/2001 08:49am
I am hoping someone can help us. We just recently began
working with paraffin embedded sections. We are getting good
staining with the primary antibodies we are using. The problem is
the counterstains. We would like to use methyl green, but we cannot
get the tissue to take it up despite our best efforts. We left it on
for an hour, we made up new methyl green, all to no avail. We are
using DAB as the chromogen for our primary antibody staining. Could
someone recommend a good counterstains that would provide nice
contrast and work in paraffin sections?
Any recommendations would be most appreciated! Thanks.
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