Counterstain for Paraffin embedded sections??

From:Kathie Berghorn <>

	I am hoping someone can help us.   We just recently began 
working with paraffin embedded sections.  We are getting good 
staining with the primary antibodies we are using.  The problem is 
the counterstain.  We would like to use methyl green, but we cannot 
get the tissue to take it up despite our best efforts. We left it on 
for an hour, we made up new methyl green, all to no avail.  We are 
using DAB as the chromogen for our primary antibody staining.  Could 
someone recommend a good counterstain that would provide nice 
contrast and work in paraffin sections?

	Any recommendations would be most appreciated!  Thanks.


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