From:Louise Taylor <>

Hi all

This is a desperate plea to anyone out there that has woked with the
antibody CATHEPSIN B
The specification sheet recomends using thyroid as a control
I find that I cannot get the control block and the test cases to work at the
same dilutions
The controls are positive at 1:100
The test cases are positive at 1:400 and 1:800
when doing the test cases at 1:100 there is too much background staining
The protocol followed is HIER WITH CITRATE BUFFER ATpH 6
Overnight incubation with primary ab
Followed with biotinylated anti-sheep at 1:200
Then Vector ABC

Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
South Africa

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