"negative controls for immunoperoxidase

<< Previous Message | Next Message >>
From:Mary Latino <latinoma@yahoo.com>
To:HistoNet Server <histonet@pathology.swmed.edu>
Content-Type:text/plain; charset=us-ascii

I am curious as to what everyone is doing for their
"negative controls" for immunohistochemistry (IHC). 
The current CAP check list, basically says that you
need a negative control slide for every Ab that you
use for each block. This seems ridiculous to me, but
only because of the way we have set up our IHC
procedures. Presently we buy prepared negative control
sera for rabbit and mouse Abs from Biogenex.  This is
probably not the best way to stain negative controls.
Presently we are manually staining with the DAKO
Envision+ system and use concentrated Abs.
We are also looking into getting an automated stainer
so the recent discussion has been a big help to us.

Mary Latino
Pathologist's Assistant
Nyack Hospital
Nyack, NY 10960  
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.

<< Previous Message | Next Message >>